| 出品公司: | Clontech |
|---|---|
| 载体名称: | pDD-tdTomato Reporter |
| 质粒类型: | 无启动子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 5351 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | DD tag (N-端） |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 新霉素（Neomycin） |
| 克隆菌株: | DH5α, HB101 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
哺乳动物载体pDD-tdTomato Reporter是研究启动子及其它顺式转录元件活性的载体； DD-tdTomato与tdTomato相比，N端融合了ProteoTuner destabilization domain (DD)去稳定结构域，导致tdTomato在细胞内很快被蛋白酶降解，降低了背景荧光，是研究启动子活性的理想报告基因。。 |
| 产品目录号: | 632193 |
| 稳定性: | 稳表达 或 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDD-tdTomato_Reporter 5351 bp DNA circular 8-MAR-2011
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 632193,632190
FEATURES Location/Qualifiers
CDS 3087..3881
/label=Kan(R)/Neo(R)
rep_origin 4466..5109
/label=pUC\origin
CDS 97..420
/label=DD
CDS 427..1854
/label=tdTomato
rep_origin complement(2107..2562)
/label=f1\origin
rep_origin 2903..3041
/label=SV40\origin
BASE COUNT 1269 a 1490 c 1460 g 1132 t
ORIGIN
1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
61 acggtaccgc gggcccggga tccaccggtc gccaccatgg gagtgcaggt ggaaaccatc
121 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc
181 gggatgcttg aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag
241 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
301 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
361 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa
421 agatctatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
481 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
541 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
601 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
661 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
721 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
781 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
841 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
901 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
961 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
1021 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
1081 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
1141 ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag
1201 gtgcgcatgg agggctccat gaacggccac gaattcgaga tcgagggcga gggcgagggc
1261 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
1321 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
1381 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
1441 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
1501 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
1561 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
1621 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
1681 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
1741 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
1801 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaggcg
1861 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
1921 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
1981 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
2041 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
2101 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
2161 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
2221 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
2281 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
2341 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
2401 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
2461 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
2521 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
2581 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
2641 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
2701 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
2761 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
2821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
2881 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
2941 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
3001 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
3061 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
3121 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
3181 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
3241 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
3301 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
3361 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
3421 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
3481 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
3541 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
3601 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
3661 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
3721 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
3781 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
3841 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
3901 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
3961 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
4021 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
4081 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
4141 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
4201 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
4261 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
4321 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
4381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
4441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
4501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
4561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
4621 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
4681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
4741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
4801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
4861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
4921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
4981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
5041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
5101 acggttcctg gccttttgct ggccttttgc tcacatgtag agaaatgttc tggcacctgc
5161 acttgcactg gggacagcct attttgctag tttgttttgt ttcgttttgt tttgatggag
5221 agcgtatgtt agtactatcg attcacacaa aaaaccaaca cacagatgta atgaaaataa
5281 agatatttta ttacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
5341 ccgccatgca t
//
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